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  • Title: Impact of short-term high-fat feeding on lipid droplet content in mouse oocytes.
    Author: Aizawa R, Ibayashi M, Hatakeyama T, Tatsumi T, Tsukamoto S.
    Journal: J Reprod Dev; 2021 Feb 15; 67(1):73-77. PubMed ID: 33071247.
    Abstract:
    Mature mammalian oocytes contain lipid droplets (LDs), which are neutral lipid storage organelles critically important for energy metabolism. In mice, maternal obesity, induced by long-term (> 3 months) high-fat feeding, contributes to the accumulation of LDs in mature oocytes. However, few studies have investigated the influence of short-term high-fat feeding on LD content. In this study, we demonstrated that 3 weeks of high-fat feeding is sufficient to increase LD content and intracellular triacylglycerol levels. Using a two-step centrifugation technique to release LDs into the perivitelline space, we found that short-term high-fat feeding increased the level of LDs in MII oocytes and that 3 days of high-fat feeding were sufficient to increase efficiency of LD release. Collectively, our study suggests that short-term high fat feeding can have a higher impact on lipid metabolism during oocyte maturation.
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