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  • Title: [Comparison of two ELISA kits for the demonstration of antibodies against LAV/HTLV-III].
    Author: Heinz FX, Kunz C, Barrett N.
    Journal: Zentralbl Bakteriol Mikrobiol Hyg A; 1987 Apr; 264(1-2):196-200. PubMed ID: 3307232.
    Abstract:
    Eight hundred sera from a non-risk group and thousand sera from people at risk for acquiring AIDS were tested for the presence of LAV/HTLV-III specific antibodies by ELISA test kits manufactured by Du Pont and Organon. Western Blot analysis was used as a confirmatory test. All Western Blot positive sera were also positive in the Du Pont ELISA which in addition revealed a very low rate of false positive results (0.44%). In the Organon ELISA negative results were obtained with one positive and one questionable positive serum, as determined by Western Blot. The possible problem of test sensitivity being too low has been taken into account by the manufacturer by changing the calculation of cut-off values.
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