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  • Title: A novel wheat germ polysaccharide: Structural characterization, potential antioxidant activities and mechanism.
    Author: Yun L, Wu T, Mao Z, Li W, Zhang M, Sun X.
    Journal: Int J Biol Macromol; 2020 Dec 15; 165(Pt B):1978-1987. PubMed ID: 33098902.
    Abstract:
    A wheat germ polysaccharide (WGP-1) was isolated from wheat germ through hot-water (pH = 7.3) extraction and further fractionated by anion-exchange column using DEAE-52 cellulose column and purified by gel-permeation chromatography using Sephacryl S-500 column. WGP-1 was mainly consisted with rhamnose, arabinose, xylose, mannose, galactose and glucose, and linked with 1→, 1 → 2, 1 → 4, 1 → 3 and 1 → 6 glucosidic bands. The present study was designed to evaluate the antioxidant activities of WGP-1 in oleic acid (OA)-induced HepG2 cell model with its related mechanisms. Results showed that WGP-1 remarkably elevated the expression of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and total antioxidant capacity (T-AOC), and decreased the levels of malondialdehyde (MDA) and reactive oxygen species (ROS) in OA-treated cell. WGP-1 was found to promote the expression of PI3K, the phosphorylation of AKT and the translocation of Nrf2, further increased the expression of HO-1 in OA-induced cells. These results demonstrate again that WGP-1 can effectively improve OA-induced oxidative damage, probably by up-regulating of PI3K expression, Nrf2 translocation, and AKT phosphorylation.
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