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  • Title: Synthesis and secretion of wheat alpha-amylase in Saccharomyces cerevisiae.
    Author: Rothstein SJ, Lahners KN, Lazarus CM, Baulcombe DC, Gatenby AA.
    Journal: Gene; 1987; 55(2-3):353-6. PubMed ID: 3311888.
    Abstract:
    A wheat alpha-amylase cDNA clone has been fused to the phosphoglycerate kinase initiator methionine to enable synthesis in the yeast Saccharomyces cerevisiae of an alpha-amylase enzyme that is identical in size to the wild-type alpha-amylase. The alpha-amylase is synthesized with an N-terminal plant signal peptide which is recognized in the yeast host, leading to efficient processing and secretion into the medium. The secretion of alpha-amylase into the medium is quite efficient in rich medium, but barely detectable in a minimal medium.
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