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  • Title: Evaluation of AOAC microbial diffusion procedure for analysis of chlortetracycline in high mineral feeds.
    Author: Katz SE, Ragheb HS, Black LB.
    Journal: J Assoc Off Anal Chem; 1987; 70(5):788-91. PubMed ID: 3316170.
    Abstract:
    The performance of the AOAC microbial diffusion assay procedure for the analysis of chlortetracycline was evaluated in mixed feeds and premixes as well as laboratory-prepared feeds, all with various mineral contents. In 100 mixed feeds with a calcium content ranging from less than 1 to greater than 22%, no relationship was shown between calcium content and incidence of chlortetracycline deficiency. In 106 premixes, a relationship was shown between chlortetracycline content and adherence to guarantee: below 10 g/lb, the incidence of deficiency was high; in premixes containing 20 g chlortetracycline and above, the incidences of deficiency were less than 5%. In laboratory-prepared feeds containing 12-15% calcium, grinding and storage at both 4 degrees C and at room temperature (20 degrees C) caused no decrease in the chlortetracycline concentration. Only storage of the ground feed at 37 degrees C caused a distinct loss of activity. The temperature-related loss was attributed to the epimerization of chlortetracycline.
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