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  • Title: Effect of GDP on the interactions between chloroplast EF-Ts and chloroplast and E. coli EF-Tu.
    Author: Spremulli GH, Spremulli LL.
    Journal: Biochem Biophys Res Commun; 1987 Nov 13; 148(3):1490-5. PubMed ID: 3318834.
    Abstract:
    The effects of varying concentrations of GDP on the stability of homologous and heterologous EF-Tu:EF-Ts complexes formed with the elongation factors from the chloroplast of Euglena gracilis and from E. coli have been investigated. The complexes formed with chloroplast EF-Ts were significantly more stable to GDP-induced dissociation than those formed with E. coli EF-Ts. The complex between chloroplast EF-Tu and chloroplast EF-Ts required nearly 1,000-fold higher concentrations of GDP for dissociation than the complex between chloroplast EF-Tu and E. coli EF-Ts. The E. coli EF-Tu:chloroplast EF-Ts complex required nearly 100-fold higher levels of GDP for dissociation than the E. coli EF-Tu:E. coli EF-Ts complex.
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