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  • Title: Development of magnetic particle-based chemiluminescence immunoassay for measurement of human procalcitonin in serum.
    Author: Liao M, Zheng J, Xu Y, Qiu Y, Xia C, Zhong Z, Liu L, Liu H, Liu R, Liang S.
    Journal: J Immunol Methods; 2021 Jan; 488():112913. PubMed ID: 33189726.
    Abstract:
    BACKGROUND: Serum procalcitonin (PCT) has been recognized as a primary biomarker in bacterial infections, and monitoring its concentration could help to evaluate the prognosis of sepsis and guide the antibiotic administration. We aimed to establish a fast and accurate immunoassay for PCT quantitation. METHODS: Our newly developed monoclonal antibodies (mAbs) against human PCT were preliminarily evaluated by enzyme-linked immunosorbent assay and then used to develop a chemiluminescence enzyme immunoassay (CLEIA). The proposed CLEIA was assessed in analytical performance and applied to measurement of serum PCT. RESULTS: mAb 2D3 and mAb 8F6 were selected as capture and detection antibody respectively, due to the highest sensitivity for PCT detection with no cross reaction to calcitonin gene-related peptides. The proposed CLEIA based on mAb pair of 2D3/8F6-AP was characterized for a working range from 0.03 to 100 μg/L. An excellent correlation was observed between our proposed assay and the VIDAS BRAHMS PCT assay (r: 0.9825). CONCLUSION: Our newly developed mAbs and CLEIA can serve as important diagnostic tools for measurement of human PCT in serum.
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