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  • Title: [Demonstration of IgE associated cells in atopic nasal mucosa using the immunoperoxidase method].
    Author: Bachert C, Ganzer U.
    Journal: Laryngol Rhinol Otol (Stuttg); 1987 Nov; 66(11):573-6. PubMed ID: 3320645.
    Abstract:
    IgE-associated cells in the specimens of 25 allergic and non-allergic subjects were stained by the peroxidase technique with the use of a polyclonal antiserum. The technique is described and its advantages over the immunofluorescence procedures are shown. In each allergic subject IgE-associated cells could be stained, whereas no cells could be found in non-atopic subjects after suppressing non-specific background staining (Fig. 4). Therefore, this method allows to distinguish between IgE-mediated allergic diseases of the nasal mucosa and diseases of other pathomechanisms. The stained cells formed a specific distribution pattern with a typical accumulation in the subepithelial and epithelial layer (Fig. 2), whereas only few cells were found in the mucosal glands.
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