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  • Title: [A method of determining the level of provitamin D in Saccharomyces cerevisiae with altered sterol metabolism].
    Author: Chichikalova MM, Zhakovskaia ZA, Moseĭchuk AV, Mikhalova NP, V'iunov KA.
    Journal: Prikl Biokhim Mikrobiol; 1987; 23(5):703-5. PubMed ID: 3321030.
    Abstract:
    A method is proposed that allows to choose among the polyene-resistant mutants of the yeasts Saccharomyces cerevisiae with changed biosynthesis of ergosterol promising producers of provitamin D4 and structural analogs of provitamin D3. The method involves both UV-spectrophotometry and thin-layer chromatography. The results obtained are supported by the data of mass-spectrometry of sterols.
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