These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Two-level factorial screening for influence of temperature, pH, and aeration on production of Serratia marcescens nuclease.
    Author: Jepsen PK, Riise E, Biedermann K, Kristensen PC, Emborg C.
    Journal: Appl Environ Microbiol; 1987 Oct; 53(10):2593-6. PubMed ID: 3322193.
    Abstract:
    Two high-nuclease-yielding mutants of Serratia marcescens, derived by chemical mutagenesis (W280, W355), and two strains with the pBR322 plasmid 403-SD2, carrying a nuclease gene and a chloramphenicol resistance gene [Escherichia coli CSH50(403-SD2) and S. marcescens CH30(403-SD2)] were investigated for nuclease production in a factorial shake flask experiment, with temperature (30 and 37 degrees C), pH (with or without CaCO3 tablets), and aeration (with or without baffles) as variable conditions. Yields varied 10-fold depending on the conditions investigated.
    [Abstract] [Full Text] [Related] [New Search]