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  • Title: Modifying the Microenvironment of Epoxy Resin to Improve the Activity of Immobilized 7α-Hydroxysteroid Dehydrogenases.
    Author: Yang Q, Li L, Wang B, Zhu L, Tan J.
    Journal: Appl Biochem Biotechnol; 2021 Apr; 193(4):925-939. PubMed ID: 33225381.
    Abstract:
    7α-Hydroxysteroid dehydrogenase (7α-HSDH) is one of the key enzymes in the catalytic reaction of taurochenodeoxycholic acid (TCDCA). To improve the activity of immobilized 7α-HSDH, the microenvironment of immobilized 7α-HSDH was modified with epoxy resin and ethanediamine (EDA). The amino-epoxy support was characterized by Fourier transform infrared (FTIR), Spectrometer elemental analysis (EA), scanning electron microscopy (SEM), contact angle (CA), and Zetasizer. The effects of the immobilization of 7α-HSDH on the amino-epoxy resin and epoxy resin were studied. The results indicated that the relative activity of immobilized 7α-HSDH on the amino-epoxy resin increased by approximately 80%. Meanwhile, the immobilized 7α-HSDH showed favorable thermal stability and operational stability. The thermal stability of immobilized 7α-HSDH increased at temperatures ranging from 15 to 35 °C, while the relative activities of 7α-HSDH immobilized on the amino-epoxy resin and epoxy resin retained 56.4% and 61.0%. After 6 cycles, the residual activities of the 7α-HSDH immobilized on the amino-epoxy resin and epoxy resin were 81.4% and 89.5%, respectively.
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