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  • Title: [Expression in Escherichia coli cells of the nucleotide sequence of DNA coding for the bovine lipotropic hormone].
    Author: Beklemishev AB, Golovin SIa, Il'ichev AA, Mamaev LV, Krasnykh VN.
    Journal: Mol Gen Mikrobiol Virusol; 1987 Oct; (10):19-23. PubMed ID: 3323897.
    Abstract:
    A cDNA fragment of bovine proopiomelanocortin coding for beta-lipotropic hormone was joined with a promoter and ribosome binding site of B. amyloliquefaciens and cloned in E. coli in pBR 327 plasmid. The level of beta-lipotropin synthesis in bacterial cells transformed by the obtained plasmid was estimated immunochemically. The level of beta-lipotropin production was shown to be 5 mg per liter of bacterial culture.
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