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  • Title: ATP-dependent RNA helicase domain of the ZC3H41 protein from Trypanosoma brucei: expression, purification and crystallization.
    Author: Izhaki-Tavor LS, Dessau M.
    Journal: Acta Crystallogr F Struct Biol Commun; 2020 Dec 01; 76(Pt 12):604-608. PubMed ID: 33263572.
    Abstract:
    A fragment of the Trypanosoma brucei ZC3H41 protein encompassing the ATP-dependent RNA helicase domain was successfully subcloned for expression in a bacterial system (Escherichia coli). Following expression, the protein was purified and crystallized using the vapor-diffusion method. The protein crystals were optimized at a 1:1 protein:reservoir solution ratio using PPGBA 2000. The optimized crystals diffracted to a dmin of 3.15 Å. The collected data revealed preliminary structural information regarding this newly discovered protein.
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