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  • Title: Neuraminidase fluorescence test for the differentiation of Trypanosoma cruzi and Trypanosoma rangeli.
    Author: Schottelius J.
    Journal: Trop Med Parasitol; 1987 Dec; 38(4):323-7. PubMed ID: 3327143.
    Abstract:
    The cell free supernatants of 13 stocks of Trypanosoma rangeli/T. rangeli-like, 50 stocks of T. cruzi/T. cruzi-like and 3 stocks of T. conorhini grown in culture were examined by using the 2-(4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid) test. It could be demonstrated that only the supernatants of the T. rangeli/T. rangeli-like stocks showed a white-yellow fluorescence under UV light. In contrast, the cell free supernatants of the T. cruzi/T. cruzi-like and T. conorhini stocks showed an ash-grey colour under UV light undistinguishable from that of the control reactions. The 4-MUAc test could be a method of choice for the routine detection of T. rangeli/T. rangeli-like flagellates in culture.
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