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  • Title: An adaptation of the Candida albicans phototoxicity test to demonstrate photosensitizer action spectra.
    Author: Gibbs NK.
    Journal: Photodermatol; 1987 Dec; 4(6):312-6. PubMed ID: 3328174.
    Abstract:
    The Candida albicans assay is widely used for the testing of potential photosensitizers, especially furocoumarins. A novel adaptation of this assay is described which provides a rapid, inexpensive and easy-to-perform method of producing action spectra. The use of filter paper rectangles with central apertures instead of the usual discs, and monochromatic irradiation with a liquid light guide reduces some of the problems associated with the standard assay. Action spectra for 8-methoxypsoralen and a new pyrrolocoumarin derivative are presented as illustrative examples.
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