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  • Title: Comparison between bloodstream and procyclic form trypanosomes for serological diagnosis of African human trypanosomiasis.
    Author: Katende JM, Nantulya VM, Musoke AJ.
    Journal: Trans R Soc Trop Med Hyg; 1987; 81(4):607-8. PubMed ID: 3328347.
    Abstract:
    Trypanosoma brucei brucei MiTat 1.2 bloodstream and corresponding procyclic forms, as well as procyclics of T. b. gambiense and T. b. rhodesiense, were fixed, in suspension, using a mixture of 80% acetone and 0.25% (v/v) formalin in saline and used as antigens for diagnosis of African human trypanosomiasis by the indirect immunofluorescent antibody test. T. b. brucei bloodstream forms detected 41/42 and 37/41 parasitologically diagnosed cases of T. b. rhodesiense and T. b. gambiense trypanosomiasis, respectively, while the procyclic stages detected the same number (41/42) of T. b. rhodesiense, but fewer (29/41) T. b. gambiense infections.
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