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Title: STUDY THE EFFECTS OF IONIZING RADIATION ON THE LEVEL OF CHROMOSOME INSTABILITY IN HUMAN SOMATIC CELLS DURING THE DEVELOPMENT OF TUMOR-INDUCED BYSTANDER EFFECT.
Author: Pilinska MA, Shemetun OV, Talan OA, Dibska OB, Kravchenko SM, Sholoiko VV.
Journal: Probl Radiac Med Radiobiol; 2020 Dec; 25():353-361. PubMed ID: 33361846.
Abstract:
OBJECTIVE: to determine the impact of the irradiated in vitro blood cells from patients with B-cell chronic lymphocytic leukemia (CLL) on the level of chromosomal instability in peripheral blood lymphocytes (PBL) from healthy persons during the development of tumor-induced bystander effect. MATERIALS AND METHODS: Separate and joint cultivation of PBL from healthy persons (cells-bystanders) together withblood cells from CLL patients irradiated in vitro at the G0 stage of the mitotic cycle by γ-quanta 137Cs in a dose of0.5 Gy 137Cs (cells-inductors) was used. For joint cultivation our own model system for co-cultivation of PBL fromindividuals of different sex, designed by us to investigate the bystander effects at the cytogenetic level was used.Traditional cytogenetic analysis of uniformly painted chromosomes with group karyotyping was performed. The frequency of chromosome aberrations in cells-inductors and cells-bystanders as the markers of chromosome instability were determined. RESULTS: Found that at co-cultivation of PBL from healthy individuals with irradiated blood cells from CLL patientsthe middle group frequency of chromosome aberrations in the bystander cells (5.18 ± 0.51 per 100 metaphases,p < 0.001) was statistically significant higher than its background level determined at a separate cultivaton (1.52± 0.30 per 100 metaphases), and at co-cultivation with non-irradiated blood cells from CLL patients (3.31 ± 0.50 per100 metaphases, p < 0.01). CONCLUSIONS: Co-cultivation of in vitro irradiated blood cells from CLL patients with PBL from healthy persons leadsto an increase in the level of chromosome instability in the bystander cells due to synergism between tumor-inducedand radiation-induced bystander effects. Meta: vyznachyty vplyv oprominenykh in vitro klityn krovi patsiientiv z V-klitynnoiu khronichnoiu limfotsytarnoiu leĭkemiieiu (KhLL) na riven' khromosomnoï nestabil'nosti v limfotsytakh peryferychnoï krovi (LPK) zdorovykh osib pryrozvytku pukhlyno-indukovanogo efektu svidka.Materialy i metody. Zastosovuvaly okreme ta spil'ne kul'tyvuvannia LPK zdorovykh osib (klityny-svidky) z klitynamy krovi patsiientiv z KhLL, oprominenymy in vitro na G0 stadiï klitynnogo tsyklu γ-kvantamy 137Cs v dozi 0,5 Gr(klityny-induktory). Dlia sumisnogo kul'tyvuvannia klityn vykorystovuvaly vlasnu model'nu systemu spil'nogokul'tyvuvannia LPK riznostatevykh osib, rozroblenu dlia doslidzhennia efektu svidka na tsytogenetychnomu rivni.Provodyly analiz rivnomirno zabarvlenykh khromosom z grupovym kariotypuvanniam. Vyznachaly chastotu khromosomnykh aberatsiĭ v klitynakh-induktorakh ta klitynakh-svidkakh.Rezul'taty. Vstanovyly, shcho pry sumisnomu kul'tyvuvanni LPK zdorovykh osib z oprominenymy klitynamy krovipatsiientiv z KhLL seredn'ogrupova chastota aberatsiĭ khromosom v klitynakh-svidkakh (5,18 ± 0,51 na 100 metafaz)statystychno znachushche perevyshchyla iak ïkh fonovyĭ riven', vyznachenyĭ pry okremomu kul'tyvuvanni (1,52 ± 0,30 na100 metafaz, p < 0,001)), tak i tseĭ pokaznyk pry ko-kul'tyvuvanni klityn-svidkiv z neoprominenymy klitynamykrovi patsiientiv z KhLL (3,31 ± 0,50 na 100 metafaz, p < 0,01).Vysnovky. Sumisne kul'tyvuvannia oprominenykh in vitro klityn krovi patsiientiv z V-klitynnoiu KhLL z limfotsytamyperyferychnoï krovi umovno zdorovykh osib pryzvodyt' do pidvyshchennia rivnia khromosomnoï nestabil'nosti vklitynakh-svidkakh vnaslidok synergizmu mizh pukhlyno-indukovanym ta radiatsiĭno- indukovanym efektamy svidka.

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