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  • Title: Heparinization of samples for plasma ionized calcium measurement.
    Author: Heining MP, Jordan WS.
    Journal: Crit Care Med; 1988 Jan; 16(1):67-8. PubMed ID: 3338281.
    Abstract:
    Maintenance of normal plasma ionized calcium concentration forms part of the management of the critically ill patient; it is, therefore, important to measure accurately plasma ionized calcium. We have compared the ionized calcium concentration of blood samples heparinized with a carefully measured amount of heparin with that of blood samples heparinized in the conventional fashion. The amount of heparin in the deadspace of a syringe was demonstrated to artifactually lower the plasma ionized calcium concentration. Samples for plasma ionized calcium measurement should be heparinized with a measured quantity of heparin, since the conventional technique introduces an unknown and probably excessive quantity of heparin into the syringe deadspace.
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