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  • Title: Quantitative determination of free amino acids by gas-liquid chromatography with special reference to yeasts.
    Author: Tunblad-Johansson I.
    Journal: Acta Pathol Microbiol Scand Suppl; 1977; (259):17-24. PubMed ID: 333865.
    Abstract:
    A gas-liquid chromatographic method for quantitative determination of free amino acids in yeasts is described. The amino acids are extracted with boiling water and separated from interfering substances on a cation-exchange resin. They are analyzed as their n-propyl N-acetyl esters. The best separation of the derivatives was achieved when using glass columns containing a mixture of three polar phases.
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