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  • Title: Giant polypeptides of skeletal muscle titin: sedimentation equilibrium in guanidine hydrochloride.
    Author: Kurzban GP, Wang K.
    Journal: Biochem Biophys Res Commun; 1988 Feb 15; 150(3):1155-61. PubMed ID: 3342063.
    Abstract:
    Titin has been purified from rabbit skeletal myofibrils in guanidine hydrochloride by gel filtration on Sephacryl S-1000. The sedimentation coefficients of denatured titin in 6 M guanidine hydrochloride are highly concentration dependent and follow the equation: 1/S = 0.241 + 0.302 A280. Equilibrium sedimentation studies indicate that the average molecular weight of titin polypeptides is 2.4 to 2.6 X 10(6).
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