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  • Title: Online sample preconcentration techniques in nonaqueous capillary and microchip electrophoresis.
    Author: John AS, Sidek MM, Thang LY, Sami S, Tey HY, See HH.
    Journal: J Chromatogr A; 2021 Feb 08; 1638():461868. PubMed ID: 33453653.
    Abstract:
    One of the major drawbacks of electrophoresis in both capillary and microchip is the unsatisfactory sensitivity. Online sample preconcentration techniques can be regarded as the most common and powerful approaches commonly applied to enhance overall detection sensitivity. While the advances of various online preconcentration strategies in capillary and microchip employing aqueous background electrolytes are well-reviewed, there has been limited discussion of the feasible preconcentration techniques specifically developed for capillary and microchip using nonaqueous background electrolytes. This review provides the first consolidated overview of various online preconcentration techniques in nonaqueous capillary and microchip electrophoresis, covering the period of the last two decades. It covers developments in the field of sample stacking, isotachophoresis, and micellar-based stacking. Attention is also given to multi-stacking strategies that have been used for nonaqueous electrophoresis.
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