These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: A non-enzymic procdure for the quantitative analysis of (3-methoxy-4-sulphoxyphenyl)ethylene glycol(MHPG sulphate) in human urine using stable isotope dilution and gas chromatography-mass spectrometry.
    Author: Murray S, Baillie TA, Davies DS.
    Journal: J Chromatogr; 1977 Nov 01; 143(6):541-51. PubMed ID: 334785.
    Abstract:
    A method is described for the quantitative analysis of (3-methoxy-4-sulphoxyphenyl)-ethylene glycol (MHPG sulphate) in human urine, based on selected ion monitoring gas chromatography--mass spectrometry and using a specifically deuterium-labelled analogue of MHPG sulphate as internal standard. The procedure involves extraction of the urine sample on Amberlite XAD-2, followed by isolation of MHPG sulphate by column chromatography on Sephadex LH-20. Cleavage of the sulphate conjugate and formation of the MHPG tris(trifluoroacetate) derivative are carried out in a one-step reaction, without recourse to enzymic hydrolysis.
    [Abstract] [Full Text] [Related] [New Search]