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  • Title: Fenton reaction-mediated dual-attenuation of signal for ultrasensitive amperometric immunoassay.
    Author: Zhang C, Zhang N, Xu Y, Feng J, Yao T, Wang F, Ma Z, Han H.
    Journal: Biosens Bioelectron; 2021 Apr 15; 178():113009. PubMed ID: 33493899.
    Abstract:
    In order to alter the complexion of immunoprobe with large impedance as negative factor in sensitivity of amperometric immunosensor, a strategy of Fenton reaction-mediated dual-attenuation of signal was proposed. Herein, metal-polydopamine-Fe3+ composite with the ability of Fenton reaction was initially prepared as immunoprobe for an ultrasensitive immunoassay. The polymerization of dopamine occurred on the surface of ZIF-67 to gain the metal-polydopamine shell, which possessed rich functional groups, negative charge and high specific surface. Then the prepared functional shell was further used to absorb Fe3+ and immobilize labeling antibody as immunoprobe, which was used to construct a sandwich type immunosensor. With addition of H2O2 and aniline, Fenton reaction was triggered to produce hydroxyl radicals, which can not only decrease the current value by degrading methylene blue molecules, but also further initiate aniline to polymerize into non-conductive polyaniline for successive abatement of signal intensity. Therefore, the dual-attenuation of signal model rendered the immunoprobe into a favorable factor and synchronously enhance sensitivity. Expectedly, the detection performance with a linear range from 1.0 × 10-4-100 ng mL-1 and ultralow detection limit of 9.07 × 10-5 ng mL-1 toward neuron-specific enolase was obtained under optimal conditions. This work offered a novel tactic for enhancing sensitivity of immunosensor through the preparation of functional immunoprobe and its rational utilization as signal enhancer.
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