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  • Title: Facilitating enzymatic hydrolysis with a novel guaiacol-based deep eutectic solvent pretreatment.
    Author: Huang C, Zhan Y, Cheng J, Wang J, Meng X, Zhou X, Fang G, Ragauskas AJ.
    Journal: Bioresour Technol; 2021 Apr; 326():124696. PubMed ID: 33508646.
    Abstract:
    Herein, we established a novel deep eutectic solvent (DES) using lignin-derived guaiacol as hydrogen bond donor (HBD). The sole ChCl/guaiacol system was found to be inefficient for the fractionation of wheat straw (WS), while the incorporation of trace AlCl3 significantly facilitated the degradation of hemicellulose and lignin, resulting in a complete enzymatic digestibility of the pretreated WS. Further, this study revealed that the DES-degraded lignin was readily precipitated during the washing process, and thus hindered the enzymatic hydrolysis of poplar and bamboo (with hydrolysis yield of 42.03% and 71.67%, respectively). Alkali washing offers a possible approach to remove the precipitated lignin, after which a near 100% hydrolysis yield was also obtained for poplar and bamboo.
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