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  • Title: Amplification and expression of heterologous ornithine decarboxylase in Chinese hamster cells.
    Author: Chiang TR, McConlogue L.
    Journal: Mol Cell Biol; 1988 Feb; 8(2):764-9. PubMed ID: 3352604.
    Abstract:
    We have developed an amplifiable mammalian expression vector based on the enzyme ornithine decarboxylase (ODC). We show greater than 700-fold amplification of this vector in ODC-deficient Chinese hamster ovary cells. A passive coamplified marker, dihydrofolate reductase (dhfr), was amplified and overexpressed 1,000-fold. This ODC vector was a dominant marker in a variety of cell types and displayed at least 300-fold amplification in wild-type Chinese hamster ovary cells.
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