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  • Title: In vitro cultivation of a Babesia isolated from a white-tailed deer (Odocoileus virginianus).
    Author: Holman PJ, Waldrup KA, Wagner GG.
    Journal: J Parasitol; 1988 Feb; 74(1):111-5. PubMed ID: 3357095.
    Abstract:
    Pyriforms and ring forms of Babesia odocoilei were detected in thin blood smears obtained from a white-tailed deer killed by a hunter in Anderson County, Texas. Erythrocytes from the deer were cultured and the parasites maintained through 8 serial subcultures during 1 mo. The parasite was successfully established in culture using Medium 199 supplemented with either 20% deer serum or 40% normal adult bovine serum. The highest parasitemia observed was 30% and more than 4 parasites per erythrocyte were often observed. Cultured B. odocoilei remained infective for a susceptible white-tailed deer.
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