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  • Title: Concanavalin A- or phorbol ester-induced translocation of protein kinase C in thymoma cells from a patient with myasthenia gravis.
    Author: Ishizuka T, Ito Y, Mori H, Miura K, Nagao S, Nagata K, Nozawa Y.
    Journal: Clin Chim Acta; 1988 Feb 29; 172(1):109-15. PubMed ID: 3359648.
    Abstract:
    We have examined the localization of phospholipid/calcium-dependent protein kinase (protein kinase C) in thymoma cells, which were resected from a patient with myasthenia gravis. Upon stimulation with 4 micrograms/ml of concanavalin A (Con A) for 30 min, protein kinase C activity in the cytosolic fraction was increased from 7.44 pmol/min per 10(7) cells to 11.42 pmol/min per 10(7) cells. On the other hand, membrane-associated protein kinase C activity was decreased from 1.69 to 0.71 pmol/min per 10(7) cells. On the contrary, 10(-7) mol/l tetradecanoyl phorbol acetate (TPA) decreased cytosolic protein kinase C activity from 9.31 to 8.04 pmol/min per 10(7) cells, while membrane-associated protein kinase C activity was increased from 1.69 to 2.94 pmol/min per 10(7) cells. Several endogenous phosphorylated proteins (mol wt 20,000, 23,000) were observed as inferred by SDS-polyacrylamide gel electrophoresis. These findings indicated that Con A and TPA produce differential translocation of protein kinase C.
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