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  • Title: Number of tRNA binding sites on 80 S ribosomes and their subunits.
    Author: Rodnina MV, El'skaya AV, Semenkov YuP, Kirillov SV.
    Journal: FEBS Lett; 1988 Apr 11; 231(1):71-4. PubMed ID: 3360133.
    Abstract:
    The ability of rabbit liver ribosomes and their subunits to form complexes with different forms of tRNAPhe (aminoacyl-, peptidyl- and deacylated) was studied using the nitrocellulose membrane filtration technique. The 80 S ribosomes were shown to have two binding sites for aminoacyl- or peptidyl-tRNA and three binding sites for deacylated tRNA. The number of tRNA binding sites on 80 S ribosomes or 40 S subunits is constant at different Mg2+ concentrations (5-20 mM). Double reciprocal or Scatchard plot analysis indicates that the binding of Ac-Phe-tRNAPhe to the ribosomal sites is a cooperative process. The third site on the 80 S ribosome is formed by its 60 S subunit, which was shown to have one codon-independent binding site specific for deacylated tRNA.
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