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  • Title: Temperature-sensitive v-sea transformed erythroblasts: a model system to study gene expression during erythroid differentiation.
    Author: Knight J, Zenke M, Disela C, Kowenz E, Vogt P, Engel JD, Hayman MJ, Beug H.
    Journal: Genes Dev; 1988 Feb; 2(2):247-58. PubMed ID: 3360325.
    Abstract:
    The isolation and characterization of a temperature-sensitive mutant (ts1 S13) of the avian erythroblastosis virus, S13, is described. The temperature-sensitive lesion in ts1 S13 was identified as affecting the tyrosine kinase activity but not the plasma membrane localization of the ts1 S13 v-sea gene product. Erythroblasts transformed by ts1 S13 can be induced to synchronously differentiate into erythrocytes in an erythropoietin (EPO)-dependent fashion. Analysis of erythrocyte-specific gene expression in ts1 S13 erythroblasts reveals that the transformed, self-renewing erythroblasts obtained at permissive temperature already express all erythrocyte genes tested for, although at a low level. Upon differentiation induction, expression of erythrocyte-specific genes is not coordinately regulated but rather involves complex regulatory mechanisms that appear to be specific for the individual genes.
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