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  • Title: Photoblueing of organic dyes can cause artifacts in super-resolution microscopy.
    Author: Helmerich DA, Beliu G, Matikonda SS, Schnermann MJ, Sauer M.
    Journal: Nat Methods; 2021 Mar; 18(3):253-257. PubMed ID: 33633409.
    Abstract:
    Illumination of fluorophores can induce a loss of the ability to fluoresce, known as photobleaching. Interestingly, some fluorophores photoconvert to a blue-shifted fluorescent molecule as an intermediate on the photobleaching pathway, which can complicate multicolor fluorescence imaging, especially under the intense laser irradiation used in super-resolution fluorescence imaging. Here, we discuss the mechanisms of photoblueing of fluorophores and its impact on fluorescence imaging, and show how it can be prevented.
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