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  • Title: Gas chromatography/mass spectrometry and gas chromatography/tandem mass spectrometry of methyl ester/methoxime/trimethylsilyl ether derivatives of prostaglandins.
    Author: Schweer H, Seyberth HW, Meese CO.
    Journal: Biomed Environ Mass Spectrom; 1988 Feb 01; 15(3):129-38. PubMed ID: 3365489.
    Abstract:
    Electron impact mass spectra of methyl ester/trimethylsilyl ether derivatives of prostaglandin F2 alpha and methyl ester/methoxime/trimethylsilyl ether derivatives of prostaglandin E2, prostaglandin D2, 6-oxo-prostaglandin F1 alpha and 2,3-dinor-6-oxo-prostaglandin F1 alpha are presented. Most of the prostaglandins studied have additionally been 2H-labelled at different sites in order to assign the corresponding fragment ions. Collisionally activated decomposition mass spectra of the most intense parent ions in the high-mass region were taken. High-intensity, prostaglandin-characteristic daughter fragments will allow a reliable quantification of prostaglandins in biological fluids and a reduction of sample clean-up.
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