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  • Title: Wave of free calcium at fertilization in the sea urchin egg visualized with fura-2.
    Author: Hafner M, Petzelt C, Nobiling R, Pawley JB, Kramp D, Schatten G.
    Journal: Cell Motil Cytoskeleton; 1988; 9(3):271-7. PubMed ID: 3365773.
    Abstract:
    A wave front of increased free calcium traversing the egg at fertilization is demonstrated in the sea urchin Lytechinus pictus. The use of the fluorescent calcium chelator fura-2 in combination with low-light-level TV microscopy and image processing allows the visualization of the Ca2+ wave front with high spatial and temporal resolution. Such a wave is demonstrated as increased fluorescence after an excitation of 340-nm wavelength and as the reciprocal image in form of a reduced fluorescence when excited at 380 nm. The band-like appearance of the wave resembles the Ca2+ wave described for larger eggs of other species. In a dispermic egg the high resolution of the system used allows us to recognize two waves of Ca2+ originating from the respective points of sperm entry.
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