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  • Title: One-step, high-yield purification of human prostatic acid phosphatase from seminal fluid by gel-filtration HPLC under nondenaturing conditions.
    Author: Rusciano D, Agostini E, Ceccarini C, Terrana B.
    Journal: Clin Chem; 1988 May; 34(5):984-6. PubMed ID: 3370802.
    Abstract:
    This is a fast, efficient method for purification to homogeneity of human prostatic acid phosphatase [orthophosphoric-monoester phosphohydrolase (acid optimum), EC 3.1.3.2], from seminal fluid. Use of a "high-pressure" liquid-chromatographic gel-filtration column permits high-yield recovery of the purified enzyme with most of its enzymatic and immunological activity retained.
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