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  • Title: The distinction of bone and liver isoenzymes of alkaline phosphatase in serum using a monoclonal antibody.
    Author: Seabrook RN, Bailyes EM, Price CP, Siddle K, Luzio JP.
    Journal: Clin Chim Acta; 1988 Mar 15; 172(2-3):261-6. PubMed ID: 3370840.
    Abstract:
    Out of 31 mouse monoclonal antibodies to human liver and bone ALP, one antibody coded BAP 1/9, showing a preference for binding the bone isoenzyme, and one antibody coded LAP 1/5, showing no preference, were selected to study binding of the serum forms of ALP. Using a plate capture assay format BAP 1/9 showed a 1.8-fold preference for binding purified bone versus liver ALP in the presence of 10% ALP-free serum compared with 2.1- to 2.25-fold preference in buffered salt solution. BAP 1/9 showed a preference for binding ALP from serum samples containing predominantly bone ALP compared to those containing predominantly liver ALP. Using predefined mixtures of serum forms of bone and liver ALP a linear relationship between ALP bound to BAP 1/9 and the fraction of bone ALP was obtained.
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