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  • Title: Isolation and partial characterization of neutrophil elastase inhibitors from bovine vitreous and aorta.
    Author: Arsenis C, Kuettner KE, Eisenstein R.
    Journal: Curr Eye Res; 1988 Feb; 7(2):95-102. PubMed ID: 3371070.
    Abstract:
    Bovine vitreous body and aorta contain extractable leukocyte elastase inhibitors, which were purified by gel filtration and affinity chromatography on agarose-pancreatic elastase. The purified inhibitor preparation from aorta was resolved by polyacrylamide gel electrophoresis into a main band migrating slightly faster than commercial Trasylol and a more weakly stained band migrating close to chymotrypsinogen. The purified inhibitor preparation from both sources inhibited, in a competitive fashion, purified human leukocyte elastase and was ineffective against bovine trypsin and leukocyte cathepsin G or collagenase. These inhibitors from vitreous body and aorta were distinguishable by several criteria from serum inhibitors.
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