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  • Title: Insertion of the tetracycline resistance translocation unit Tn10 in the lac operon of Escherichia coli K12.
    Author: Foster TJ.
    Journal: Mol Gen Genet; 1977 Sep 09; 154(3):305-9. PubMed ID: 337110.
    Abstract:
    The majority of TN10 insertions in the lacZ gene of Escherichia coli occurred in a small region of the promoter distal part of the gene. The resulting mutations were polar on lacY and reverted to Lac+ at a frequency of 10(-8). None of the revertants were Tcr. Furthermore Lac+ Tcr revertants could not be selected directly. Relief of polarity revertants of the lacZ::Tn10 mutants were formed at a frequency of 10(-5) - 10(-4). Most resulted from a deletion event internal to the transposon which removed the Tcr genes and the putative transcription terminator. It is postulated that a fragment of Tn10 remains at the original insertion point to cause a revertible Lac- mutation.
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