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  • Title: Determination of phylloquinone and menaquinone in human milk using high performance liquid chromatography.
    Author: Isshiki H, Suzuki Y, Yonekubo A, Hasegawa H, Yamamoto Y.
    Journal: J Dairy Sci; 1988 Mar; 71(3):627-32. PubMed ID: 3372812.
    Abstract:
    A high performance liquid chromatographic method for measuring vitamin K in human milk and cow milk is described. The K vitamins were extracted with n-pentane from enzymatic hydrolysate of milk, purified by semipreparative HPLC, and then analyzed by reversed-phase HPLC equipped with a dual electrochemical detector. The amount of phylloquinone and menaquinone-4 in human milk was 2.1 +/- .9 and 1.3 +/- 1 microgram/L, respectively (n = 23). A small amount of menaquinone-6 was detected in both human and cow milk.
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