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  • Title: An improved purification method for cytosolic malate dehydrogenase from several sources.
    Author: Domènech C, Abante JJ, Bozal FX, Mazo A, Cortés A, Bozal J.
    Journal: Prep Biochem; 1988; 18(1):17-35. PubMed ID: 3375202.
    Abstract:
    A new purification method for cytosolic malate dehydrogenases from several sources has been developed. The procedure, employing chromatographies on 5'AMP-Sepharose, DEAE-Sephacel and Blue-Sepharose, allows for a rapid isolation of the enzyme (approximately 40 hours), in large quantities, with good yields (45-54%). The specific activity of final preparations were around 1300 I.U./mg and were judged homogeneous by polyacrylamide gradient gel and sodium dodecyl sulfate polyacrylamide gel electrophoresis, high performance size exclusion chromatography and isoelectric focusing.
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