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Title: [Effects of electroacupuncture on pain behavior and pain-related factors in spinal cord dorsal horn and dorsal root ganglia of rats with knee osteoarthritis].
Author: Yang KW, Yuan PW, Dong B, Fan JJ, Wang YY, Liu H, Chai X, Zheng J.
Journal: Zhen Ci Yan Jiu; 2020 Oct 25; 45(10):818-22. PubMed ID: 33788448.
Abstract:
OBJECTIVE: To observe the effect of electroacupuncture(EA) on the pain behavior and prostaglandin E2 (PGE2), calcitonin gene-related peptide (CGRP) and substance P (SP) in the spinal cord dorsal horn and dorsal root ganglia (DRG) of rats with knee osteoarthritis (KOA), so as to explore the mechanisms of EA underlying improvement of chronic pain in KOA rats. METHODS: Thirty-two female SD rats were randomly divided into blank group, control group, model group and EA group, with 8 rats in each group. Rats in the control group were injected with 50 μL of 0.9% sodium chloride solution into the left knee joint cavity, and rats in the model and EA groups were injected with 50 μL of Monosodium iodoacetate in the left knee joint. EA(2 Hz/100 Hz, ＜2 mA) was applied to left "Yanglingquan"(GB34) and "Neixiyan" (EX-LE4) for 15 min, once daily, 5 days a course with a total of 2 courses. Paw withdrawal latency (PWL) and mechanical pain threshold (PWT) were tested by Plantar Test and Von Frey, separately. After the last pain test, the contents of PGE2, CGRP and SP in the left lumbar (L) 3-L5 DRG and L3-L5 spinal dorsal horn were detected by ELISA. RESULTS: Compared with the blank group, the PWL and PWT of the rats in the model group were significantly decreased (P<0.01), and the contents of PGE2, CGRP and SP in the DRG and spinal dorsal horn were significantly increased (P<0.01, P<0.05). There were no statistically significant differences in PWL, PWT, contents of PGE2, CGRP and SP in DRG and spinal dorsal horn between the blank group and the control group (P>0.05). Compared with the model group, the PWL and PWT of rats in the EA group were significantly increased (P<0.01), and the contents of PGE2, CGRP and SP in the DRG and spinal dorsal horn were significantly decreased (P<0.01, P<0.05). CONCLUSION: EA of GB34 and EX-LE4 can reduce the levels of pain-related factors PGE2, CGRP and SP in the DRG and spinal dorsal horn, thereby relieving spinal hyperalgesia in rats with KOA. 目的：观察电针对膝骨性关节炎(KOA)大鼠痛行为及脊髓背角和背根神经节(DRG)中前列腺素E2(PGE2)、降钙素基因相关肽(CGRP)和P物质(SP)含量的影响，探讨电针治疗KOA慢性疼痛的机制。方法：将32只雌性SD大鼠随机分为空白组、对照组、模型组和电针组，每组8只。对照组大鼠予左膝关节注射50 μL 0.9%氯化钠溶液，模型组和电针组大鼠予左膝关节注射50 μL谷氨酸钠碘乙酸进行造模。电针组于造模后14 d予大鼠左侧“阳陵泉”和“内膝眼”电针干预15 min，每日1次，5次为1个疗程，共治疗2个疗程。对大鼠进行行为学测定，包括缩爪潜伏期(PWL)和机械性痛阈值(PWT)。最后一次测痛结束后用ELISA法检测大鼠左侧腰(L)3—L5 DRG和脊髓背角中PGE2、CGRP、SP的含量。结果：与空白组比较，造模后模型组大鼠的PWL、PWT明显降低(P<0.01)，DRG和脊髓背角中PGE2、CGRP和SP含量显著升高(P<0.01，P<0.05)；对照组大鼠PWL、PWT, DRG和脊髓背角中PGE2、CGRP和SP的含量差异无统计学意义(P>0.05)。与模型组比较，电针干预后，电针组大鼠的PWL、PWT显著升高(P<0.01)，DRG和脊髓背角中PGE2、CGRP和SP含量显著降低(P<0.01，P<0.05)。结论：电针“阳陵泉”“内膝眼”可减少KOA大鼠疼痛相关因子PGE2、CGRP和SP的生成，从而降低大鼠脊髓痛觉敏化水平，缓解KOA导致的疼痛。.

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