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Title: [Effect of electroacupuncture on angiopoietin-1 in spinal cord of rats with neuropathic pain].
Author: Wu QY, Zhou KC, Yue JJ, Tu WZ, Yang GH, Jiang SH.
Journal: Zhen Ci Yan Jiu; 2021 Mar 25; 46(3):209-14. PubMed ID: 33798293.
Abstract:
OBJECTIVE: To observe the effect of electroacupuncture (EA) on the behavior, histomorphology and the expression of angiopoietin-1 (Angpt-1) in rats with spinal nerve injury, so as to explore its mechanism on neuropathic pain. METHODS: Forty-five male SD rats were randomly divided into sham, model and EA groups (n=15 rats in each group). Spinal nerve ligation (SNL) of the L5 lumbar vertebra was performed to establish a rat model of neuropathic pain. The rats in the EA group were given EA at "Zusanli" (ST36) and "Kunlun" (BL60) of the operation side with continuous wave at a frequency of 2 Hz and an intensity of 1.5 mA once a day, 30 minutes each time for 7 days. The sham group only exposed L5 spinal nerves without ligation. Mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) were observed and recorded before modeling and on days 3,5,7,10,12 and 14 after modeling. L4-L6 segments of spinal cord were taken and the morphological changes of spinal dorsal horn were observed by HE staining. The changes of spinal dorsal horn nerve fiber structure were observed by silver plating staining. Angpt-1 expression was detected by Western blot and immunohistochemistry. RESULTS: Compared with the sham group, the model group had significant reductions in MWT and TWL at each time point (P<0.01); compared with the model group, the EA group had significant increases in MWT and TWL on days 10,12 and 14 after intervention (P<0.05, P<0.01). HE staining showed that in the model group, the spinal dorsal horn showed degeneration and necrosis of neurons, nuclear fixation and shrinkage, and loose surrounding tissues. The degree of tissue damage of the EA group was milder than that of the model group. The silver staining results showed the model group had obvious neuronal fibrillary tangles, while there were fewer neuronal fibrillary tangles in the EA group. Compared with the sham group, the Angpt-1 expression in the model group was significantly decreased (P<0.01), and compared with the model group, the EA group had a significant increase in the expression of Angpt-1 (P<0.01). CONCLUSION: EA can promote the recovery of nerve function in SNL rats by up-regulating Angpt-1 expression. 目的：观察电针对脊神经损伤（SNL）大鼠行为学、脊髓背角组织形态学及脊髓血管生成素-1(Angpt-1)表达的影响，探讨电针治疗神经病理性疼痛的作用机制。方法：雄性SD大鼠随机分为假模组、模型组、电针组，每组15只。假模组仅暴露右侧腰(L)5脊神经，不结扎；模型组、电针组结扎右侧L5脊神经建立神经病理性疼痛大鼠模型。电针组于术后第7天开始电针“足三里”“昆仑”，每次30 min，1次/d，连续电针7 d。术后观察并记录大鼠患侧机械缩足反射阈值(MWT)和足底热缩足反射潜伏期(TWL)。采用HE染色法观察大鼠患侧L4—L6段脊髓背角形态学的改变；镀银染色法观察脊髓背角神经纤维结构的变化；Western blot及免疫组织化学法检测脊髓背角Angpt-1表达水平。结果：与同时点假模组比较，模型组大鼠患侧MWT、TWL明显降低(P<0.01)；电针组术后第10、12、14天的MWT、TWL明显高于同时点模型组(P<0.05，P<0.01)。假模组大鼠脊髓背角组织结构未见明显异常，神经纤维数量多，结构清晰；模型组脊髓背角可见神经元变性坏死、核固缩、周围组织疏松，神经纤维缠结，数量减少；电针组组织损伤程度低于模型组，神经纤维缠结较少，数量增多。模型组大鼠脊髓背角Angpt-1蛋白表达明显低于假模组(P<0.01)；电针组Angpt-1蛋白表达明显高于模型组(P<0.01)。结论：电针可通过上调Angpt-1的表达促进SNL大鼠神经功能的恢复。.

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