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  • Title: Isolation of alpha-crystallin and its subunits by affinity chromatography on immobilized monoclonal antibodies.
    Author: Stevens A, Augusteyn RC.
    Journal: Exp Eye Res; 1988 Apr; 46(4):499-505. PubMed ID: 3384010.
    Abstract:
    Use has been made of the specific interactions between monoclonal antibodies and the alpha A or alpha B subunits of alpha-crystallin to devise methods for the purification of the intact protein or its subunits. alpha A and alpha B subunits were separated by affinity chromatography on an immobilized monoclonal antibody specific for alpha A chains, using a pH gradient. Use of an antibody which binds both subunits has enabled the isolation of intact alpha-crystallin aggregates. Gel electrophoresis, conformational probing and size analysis showed that the affinity purified proteins were purer but otherwise indistinguishable from the alpha-crystallins isolated by other methods.
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