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  • Title: Crystallization of cytochrome P-450scc from bovine adrenocortical mitochondria.
    Author: Iwamoto Y, Tsubaki M, Hiwatashi A, Ichikawa Y.
    Journal: FEBS Lett; 1988 Jun 06; 233(1):31-6. PubMed ID: 3384090.
    Abstract:
    Cytochrome P-450scc (P-450scc), a cholesterol side-chain cleavage enzyme from bovine adrenocortical mitochondria, has been crystallized for the first time. Upon removal of glycerol from the solution of the native enzyme complexed with pyridoxal 5'-phosphate (PLP) by microdialysis against distilled water, reddish and planar crystals appeared. The crystals of native P-450scc were also obtained by the same procedure. We identified the crystals as the P-450scc-PLP complex or native P-450scc by absorption spectroscopy and SDS-polyacrylamide gel electrophoresis, and characterized them under a polarization microscope.
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