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  • Title: Cloning and amplification of alpha and beta mouse globin gene sequences synthesised in vitro.
    Author: Rougeon F, Mach B.
    Journal: Gene; 1977 May; 1(3-4):229-39. PubMed ID: 338418.
    Abstract:
    New chimeric Escherichia coli plasmids containing alpha or beta globin gene sequences of the mouse were constructed. Double-stranded DNA, synthesised in vitro in a 2-step reaction from mouse globin mRNA was inserted into E. coli plasmid pCR1, after tailing of the 2 DNAs with dG and dC respectively. Some of the mouse globin plasmids described contain at least 90% of the globin mRNA sequence and therefore contain the entire translated sequence of the globin genes. Some possible uses of these recombinant plasmids are described.
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