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  • Title: Rapid, simultaneous determination of isomers of retinal, retinal oxime and retinol by high-performance liquid chromatography.
    Author: Landers GM, Olson JA.
    Journal: J Chromatogr; 1988 Apr 22; 438(2):383-92. PubMed ID: 3384888.
    Abstract:
    Thirteen geometric isomers of retinol, retinal and retinal oxime were resolved in a single chromatographic run on two 250 X 4 mm 5-micron LiChrosorb Si 60 columns in series, using 11.2% ethyl acetate, 1.4% 1-octanol and 2% dioxane in hexane as the mobile phase. All the 11-cis and all-trans isomers of retinol and retinal oxime were completely resolved from each other and from the 9-cis and 13-cis isomers. This chromatographic procedure should be particularly useful for quantifying geometric isomers of retinoids in methylene chloride-hydroxylamine extracts of biological samples such as ocular tissues.
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