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  • Title: A hybrid ampholyte focusing technique for esterase D subtyping of evidentiary material.
    Author: Budowle B, Gambel AM.
    Journal: J Forensic Sci; 1988 May; 33(3):738-43. PubMed ID: 3385383.
    Abstract:
    An ultrathin-layer polyacrylamide gel isoelectric focusing technique that uses a composite of ampholytes from three commercial sources is described for subtyping esterase D. All common allelic products of esterase D were separated clearly. The technique described in this paper provides a higher conclusive call rate on known blood specimens (95.8%) and questioned bloodstains (69.7%) compared with continuous zone electrophoresis in agarose gels (89.9 and 37.6%, respectively).
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