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  • Title: Indirect Detection of Ligand Binding by Thermal Melt Analysis.
    Author: Shaw J, Stubbs C.
    Journal: Methods Mol Biol; 2021; 2263():201-215. PubMed ID: 33877599.
    Abstract:
    A thermal shift assay (TSA) involves measuring the effect of a compound on the thermal stability of a protein as an indirect measure of ligand binding. In this chapter, we provide a protocol for a conventional TSA with recombinant/purified proteins using differential scanning fluorimetry (DSF), followed by a protocol for a Cellular Thermal Shift Assay (CETSA®), which measures the soluble cellular protein remaining after a transient heat shock of live cells to detect intracellular ligand binding.
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