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  • Title: [Effect of a varying body allowance of protein and essential amino acids on the pool of free amino acids in the blood and tissues].
    Author: Mukhamedzhanov EK.
    Journal: Vopr Pitan; 1988; (2):27-32. PubMed ID: 3388806.
    Abstract:
    The pool of free amino acids in the blood and tissues was studied, basing on the protein amount in the diet and its qualitative composition, in 60 male WAG rats (bw 130-150 g), using gas-liquid chromatography. Three levels of casein were used in the ration (8.25 and 64% by mass). Incomplete protein of vegetable origin--wheat gluten was given instead of casein in an amount of 25%. The data obtained evidence that alanine circulation in deficiency of protein and essential amino acids is lowered which is associated with the inhibition of transamination of branched amino acids into glutamate. Deficiency of lysine, methionine, threonine and tryptophan in wheat gluten, when it is given to rats, leads to a decrease of these amino acids' pool in the skeletal muscles and blood that indicates the development of their deficiency in the body. The use of the relevant protein in the animals' diet resulted in disorders oi gluconeogenic amino acids (serine, glycine, proline), aspartate and cysteine metabolism. The protein excess leads to the growth of most amino acids' pool. Thus, metabolism of amino acids and their content in the tissue depend on the direction of the amino acid metabolic processes and their concentration in the food ration.
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