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  • Title: Immunohistochemical and ultrastructural identification of the pancreatic polypeptide-producing cell (PP-cell) in the human pancreas.
    Author: Baetens D, De Mey J, Gepts W.
    Journal: Cell Tissue Res; 1977 Dec 13; 185(2):239-46. PubMed ID: 340043.
    Abstract:
    The consecutive semithin--thin section method was employed to identify pancreatic-polypeptide-containing cells in the human pancreas. The immunocytochemical staining of semithin sections with the peroxidase-antiperoxidase complex allowed a precise ultrastructural description of the immunoreactive cells on the adjacent thin section. Two populations of small granulated cells could be distinguished with this technique: one group is formed by cells whose secretory granules with a mean diameter of 141 nm show specific immunoperoxidase staining with anti-PP serum, while the other group consists of cells whose secretory granules measuring 118 nm in diameter show no immunoreactivity to anti-PP serum. The former cells which will be designated as PP-cells, are not identical with the D1-cells of the revised Wiesbaden classification of endocrine cells.
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