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  • Title: Methylcitric acid determination in amniotic fluid by electron-impact mass fragmentography.
    Author: Kretschmer RE, Bachmann C.
    Journal: J Clin Chem Clin Biochem; 1988 May; 26(5):345-8. PubMed ID: 3404093.
    Abstract:
    Methylcitric acid, a metabolite of abnormal propionyl-CoA metabolism, is elevated in amniotic fluid when the fetus is affected with propionic acidaemia or methylmalonic aciduria. We developed a method for quantifying methylcitric acid in amniotic fluid by solid-phase extraction, derivatisation to the t-butyl-dimethylsilylester, and gas chromatography-mass spectrometry with electron-impact ionisation, using the deuterated analogue of methylcitric acid as an internal standard. The main advantages are a good sensitivity, simple sample preparation, and feasibility on instruments equipped with mass specific detectors.
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