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  • Title: The detection, purity and structural properties of partially soluble mushroom and cereal β-D-glucans: A solid-state NMR study.
    Author: Kremmyda A, MacNaughtan W, Arapoglou D, Eliopoulos C, Metafa M, Harding SE, Israilides C.
    Journal: Carbohydr Polym; 2021 Aug 15; 266():118103. PubMed ID: 34044921.
    Abstract:
    β-D-glucans are proposed to have many health benefits. It is therefore important to have methods which can distinguish these from other carbohydrates present in natural products, as well as giving glucan content and structural information. Correlations between features in the CP/MAS spectra of β-D-glucans and enzyme assay determined β-D-glucan content were generally found to be poor. The β-D-glucan in dry and hydrated forms of the mushroom Ganoderma lucidum was investigated in detail by spectral peak fitting to the anomeric carbon C1 region in CP/MAS NMR spectra. Hydrated samples gave spectra with enhanced resolution and suggested that a clear distinction between β-D-glucans and other carbohydrates could be possible in the anomeric carbon C1 region. Chemical shift values for a range of carbohydrate polymers, which can be found alongside β-D-glucans, as well as the values for various linkages are given. Contamination by other carbohydrates and buffer salts is discussed.
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